The release of nanoparticles into the environment can interfere with the health of the exposed organisms.MicroRNAs have been suggested as potential toxicology biomarkers.The expression of potential zebrafish nano-toxicity biomarker miRNAs in our previous study was validated in THP-1 human monocytic cell line after exposure to polystyrene (PSNPs) and ARS labeled Titanium dioxide nanoparticles (nano-TiO2-ARS).miRNAs expression post exposure to PLGA nanoparticles and E.
coli BioParticles was used to exclude bostik roll-cote potential activation and engagement of miRNAs through phagocytosis or pro-inflammatory specific responses.miR-155-5p showed the highest potential to be used as biomarker for PSNPs and nano-TiO2-ARS induced toxicity.To determine effects of PSNPs and nano-TiO2-ARS on genotoxicity, time and dose dependent DNA damage profile was established.Severe DNA damage was triggered by both nanoparticles, and expression of DNA damage repairing genes was elevated post nano-TiO2-ARS, but not post PSNPs exposure, questioning the utility of the comet assay as universal assessment tool for genotoxicity induced by nanoparticles in general.
Transfection of miR-155-5p mimic influenced read more the expression of miR-155-5p related, DNA damage responsible genes post both nano-TiO2-ARS and PSNPs exposure.Transfection results suggest significant involvement of miR-155-5p in gene repair mechanisms triggered by adverse effects of PSNPs and nano-TiO2-ARS on monocytes.